Professor Hoi Shan KWAN, PhD, BBS, JP

Address (Office): Rm 288, Science Center, CUHK
(Lab): Rm 377, Science Center, CUHK
Phone (Office): (852)3943 6285
(Lab): (852)3943 6270
Fax: (852)39431146, (852)39435745
Email: hoishankwan@cuhk.edu.hk
hskwan@eservices.cuhk.edu.hk
Web: http://kwanlab.bio.cuhk.edu.hk/

Education
1982   PhD (Microbiology), University of California, Davis, USA
1978   MPhil (Biology), The Chinese University of Hong Kong,      Hong  Kong
1977    PG Dip Microbiol Biotech (Microbiology and Biotechnology), Osaka & Kyoto University, Japan
1975    BSc (Hon.) (Biology), The Chinese University of Hong Kong, Hong Kong

Honours

2012 Bronze Bauhinia Star, Hong Kong SAR

2007 Justice of the Peace, Hong Kong SAR

Positions

1997-present Professor (School of Life Sciences, The Chinese University of Hong Kong, Hong Kong)
2008-present Director (Food Research Centre, The Chinese University of Hong Kong, Hong Kong)
2009-present Member, Hong Kong Council for Testing and Certification, Innovation and Technology Commission, HKSAR
2006-2012 Chairman, Expert Committee on Food Safety, Food and Environmental Hygiene Department, HKSAR
2006-2012 Chairman, Accreditation Advisory Board, Hong Kong Accreditation Service, Innovation and Technology Commission, HKSAR
2004-present Member, Scientific Committee on Enteric Infections and Foodborne Diseases, Centre for Health Protection, Department of Health, HKSAR
1998-present Member (Election Committee for 9th, 10th & 11th National People’s Congress of People’s Republic of China, Hong Kong SAR, PRC)
1999-present Professor (by Courtesy) (School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong)
2006-present Deputy Director (Centre for Food and Drug Safety, The Chinese University of Hong Kong, Hong Kong)
2006-2009 Dean (Faculty of Science, The Chinese University of Hong Kong, Hong Kong)
2005-2006 Acting Dean (Faculty of Science, The Chinese University of Hong Kong, Hong Kong)
2003-2005 Associate Dean (Faculty of Science, The Chinese University of Hong Kong, Hong Kong)
2003-2005 Director (Food and Nutritional Sciences Programme, The Chinese University of Hong Kong, Hong Kong)
2002-2005 President (The Teachers’ Association of the Chinese University of Hong Kong, Hong Kong)
2001-2007 Member (Biology and Medicine Panel, Research Grants Council, University Grants Committee, Hong Kong SAR Government)
2000-2003 Director (Molecular Biotechnology Programme, The Chinese University of Hong Kong, Hong Kong)
2000-2007 Member (Advisory Council on Food and Environmental Hygiene, Health, Welfare and Food Bureau, Hong Kong SAR Government)
2000-2007 Member (Publicity and Public Education Group on Clean Hong Kong, Environment and Food Bureau, Hong Kong SAR Government)
2000-2007 Member (Steering Committee on Clean Hong Kong, Environment and Food Bureau, Hong Kong SAR Government)
1999-2000 Associate Director (Molecular Biotechnology Programme, The Chinese University of Hong Kong, Hong Kong)
1998-2006 Member (Election Committee for Legislative Council and Chief Executive, Hong Kong SAR Government)
1998-present Visiting Professor in Microbiology (Microbiology, Naikai University, PRC)
1996-1997 Associate Professor (Dept of Biology, The Chinese University of Hong Kong, Hong Kong)
1995-1999 Head (Biology Graduate Division, Dept of Biology, The Chinese University of Hong Kong, Hong Kong)
1995-1996, 1997-1998, 1999-2002 Associate Director (Food and Nutritional Sciences Programme, The Chinese University of Hong Kong, Hong Kong)
1994-1995, 1996-1998 Director (Food and Nutritional Sciences Programme, The Chinese University of Hong Kong, Hong Kong)
1994-1996 Senior Lecturer (Dept of Biology, The Chinese University of Hong Kong, Hong Kong)
1992-1993 Visiting Assistant Professor (Microbiology & Molecular Genetics, Harvard Medical School, USA)
1991-1993 Dean of Students (United College, The Chinese University of Hong Kong, Hong Kong)
1991-1992,
1997-present Director (Research Laboratory of Food Science, The Chinese University of Hong Kong, Hong Kong)
1989-1991 Director (Research Laboratory for Food Protein Production, The Chinese University of Hong Kong, Hong Kong)
1988-present Fellow (Assembly of Fellows, United College, The Chinese University of Hong Kong, Hong Kong)
1984-1993 Lecturer (Dept of Biology, The Chinese University of Hong Kong, Hong Kong)
1983-1984 Postdoctoral Research Bacteriologist (Department of Bacteriology, University of California, Davis, USA)
1982 Postdoctoral Research Microbiologist (Department of Food Science and Technology, University of California, Davis, USA)
1979-1982 Associate in Food Science & Technology (Department of Food Science and Technology, University of California, Davis, USA)
1975-1976,
1977-1978 Student Demonstrator (Dept of Biology, The Chinese University of Hong Kong, Hong Kong)

Research Interests

Genomics and functional genomics
Molecular genetics and genomic analysis of bacteria, fungi and marine animals
Food Safety and Authentication
Microbiology
Molecular Biology and Biotechnology
Molecular Analysis of Chinese Medicine and Food

Representative Publications

  • Chum WW, Kwan HS, Au CH, Kwok IS, Fung YW. 2011. Cataloging and profiling genes expressed in Lentinula edodes fruiting body by massive cDNA pyrosequencing and LongSAGE. Fungal Genetics and Biology 48:359-369.
  • Stajich JE, Wilke SK, Ahrén D, Au CH, Birren BW, Borodovsky M, Burns C, Canbäck B, Casselton LA, Cheng CK, Deng J, Dietrich FS, Fargo DC, Farman ML, Gathman AC, Goldberg J, Guigó R, Hoegger PJ, Hooker JB, Huggins A, James TY, Kamada T, Kilaru S, Kodira C, Kües U, Kupfer D, Kwan HS, Lomsadze A, Li W, Lilly WW, Ma LJ, Mackey AJ, Manning G, Martin F, Muraguchi H, Natvig DO, Palmerini H, Ramesh MA, Rehmeyer CJ, Roe BA, Shenoy N, Stanke M, Ter-Hovhannisyan V, Tunlid A, Velagapudi R, Vision TJ, Zeng Q, Zolan ME, Pukkila PJ. 2010. Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proceedings of the National Academy of Sciences of the United States of America 107:11889-11894.
  • Cheung MK, Au CH, Chu KH, Kwan HS, Wong CK. 2010. Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing. The ISME Journal 4:1053-1059.
  • Chum, W.W.Y., Ng K.T.P., Shih, R.S.M., Au, C.H. and Kwan, H.S.2008. Gene expression studies of the dikaryotic mycelium and primordium of Lentinula edodes by serial analysis of gene expression.  Mycological Research 12:950-964.
  • Szeto C.Y.Y., Wong Q.W.L., Leung G.S. and Kwan H.S.2008. Isolation and transcript analysis of two-component histidine kinase gene Le.nik1 in Shiitake mushroom, Lentinula edodes. Mycological Research 112:108-116.
  • Mak, C.Y., Cheung, K.S., Yip, P.Y. and Kwan, H.S.2008. Molecular evidence for the hybrid origin of Bauhinia blakeana (Caesalpinioideae). Journal of Integrative Plant Biology 50:111-118.
  • Yip, P.Y., Chau, C.F., Mak, C.Y. and Kwan, H.S.2007. DNA methods for identification of Chinese medicinal materials. Chinese Medicine 5:2-9.
  • Lee, M.T., Szeto C.Y.Y., Ng, T.P. and Kwan, H.S.2007. Endocytosis in the Shiitake mushroom Lentinula edodes and the involvement of GTPase Le.RAB7. Eukaryotic Cell 6:2406-2418.
  • Szeto, C. Y.Y., Leung, G.S. and Kwan, H.S.2007. Le.MAPK and its interacting partner, Le.DRMIP, in fruiting body development in Lentinula edodes. Gene 393:87-93.
  •  Kwan, H.S.and Xu, H.L. 2002. Construction of a genetic linkage map of Shiitake mushroom Lentinula edodes strain L-54. Journal of Biochemistry and Molecular Biology 35:465-471.

Research Grant

  • 2012-2015, Research Grants Council (RGC) General Research Fund, $800,000
  • 2011-2013, Innovation and Technology Fund (ITF), First-stage development of platform for authentication of dried seafood and tonic food products, $2,999,959
  • 2011-2012, Knowledge Transfer Project Fund, Virtual dietician at 1 touch – develop an apple application on the 1st Hong Kong commonly used food and ingredient database for portable handheld users, $200,000
  • 2010-2012, CUHK Research Committee Funding (Direct Grants), Genome sequencing and identification of microatellite markers in the Shitake mushroom Lentinula edodes for biological studies and horticultural applications, $46,443
  • 2010-2011, Food and Environmental Hygiene Dept, HKSAR Government, Provision of service for sampling and food preparation for the first Hong Kong total diet study, $1,238,320
  • 2009, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Genome sequencing of Lentinula edodes 135, $150,000
  • 2009-2011, Research Fund for the Control of Infectious Diseases (RFCID), Metagenomics of Tuberculosis infection in Hong Kong, $800,000
  • 2008-2010, CUHK Research Committee Funding (Direct Grants), Transciptome analysis of fruiting body initiation of the basidiomycete mushroom Coprinopsis cinerea, $38,680
  • 2008-2010, CUHK Research Committee Funding (Direct Grants), Transcriptome analysis of early sclerotium development in Coprinoprsis cinerea, $30,000
  • 2008-2010, Research Fund for the Control of Infectious Diseases (RFCID), Complete genome analysis of drug-susceptible and multidrug-resistant Mycobacterium Tuberculosis Beijing family strains, $798,648
  • 2008-2011, Research Grants Council (RGC) General Research Fund, Transcriptome and gene functions analyses of mushroom fruiting body development in Coprinopsis Cinerea by 5′ SAGE, RNAi silencing, immunostaining and microscopy, $1,001,887
  • 2008-2010, Innovation & Technology Commission-Innovation and Technology Support Programme, A yeast expression system for the commercial production of recombinant ligninolytic enzymes from edible Shiitake mushrooms, $874,875
  • 2008-2011, Research Fund for the Control of Infectious Diseases (RFCID) Commissioned Grant, First-stage development of a comprehensive genome sequence database for the identification of foodborne pathogens in Hong Kong, $6,176,045
  • 2008-2013, Focused Investments Scheme of CUHK, Establishment of the Centre for Microbial Genomics and Proteomics, $10,000,000
  • 2008-2010, Focused Investments Scheme of CUHK, Hong Kong Bioinformatics Centre, $1,508,000
  • 2008-2009, Government Laboratory, HKSAR Government, Amplicon DNA library preparation and sequencing for 2 samples, $49,000
  • 2007-2010, Research Grants Council (RGC) Earmarked Grants,Role of the brownish shiitake mycelia exudate on the cell death of Leukemia cell line and its role as an antioxidant and a functional food colorant, $920,500
  • 2007-2009, CUHK Research Committee Funding (Direct Grants), Molecular population structure of squaretail coral grouper in the Indo-Pacific, $38,680
  • 2007-2009, CUHK Research Committee Funding (Direct Grants), Isolation and in silico analysis of promoters from Shiitake Mushroom Lentinula edodes, $39,000
  • 2007-2009, CUHK Research Committee Funding (Direct Grants), Gene expression studies on different development stages of a novel fungus, Pleurotus Tuber-regium, $80,000
  • 2007-2008, CUHK Research Committee Funding (Direct Grants), Mechanism of immunomodulatory and antitumor activity of structurally characterized mushroom sclerotial ß-glucans: cell surface receptor and cell signaling, $38,680
  • 2006-2007, CUHK Research Committee Funding (Direct Grants), Mechanism of immunomodulatory and antitumor activity of structurally characterized Mushroom sclerotial ß-glucans: cell surface receptor and cell signaling, $39,000
  • 2006-2007, Dept of Health, HKSAR Government, Research and laboratory work on three selected herbs, namely Cortex mori, Cortex eucommiae, and Fructus evodiae, $1,280,000
  • 2006-2007, Agriculture, Fisheries & Conservation Department, HKSAR Government, Genetic diversity analysis of Cuora trifasciata and its allied species using microsatellite markers developed from Mauremys rivulata, $50,000
  • 2006-2011, The Hong Kong Jockey Club Charities Trust – Chief Executive Community Project List 2006,Construction of food composition database, $17,770,000
  • 2006-2008, CUHK Research Committee Funding (Direct Grants), Isolation, characterization and application of conditionally expressed promoters from Shiitake mushroom Lentinula edodes, $54,125
  • 2005-2008, Research Grants Council (RGC) Earmarked Grants,Mushroom non-digestible carbohydrates as novel β-glucan type prebiotics: their preparation and in vitro microbial fermentation, $718,957
  • 2005-2007, CUHK Research Committee Funding (Direct Grants), Genes and molecular markers involved in the control of erect flag leaf in rice, $54,735
  • 2005, Agriculture, Fisheries & Conservation Dept, HKSAR Government, DNA sequencing and genetics diversity analysis, $50,000
  • 2004-2005, Dept of Health, HKSAR Government, Research and laboratory work on the Chinese medicinal herb, namely Rhizoma Cimicifugae, $381,350
  • 2004-2005, Dept of Health, HKSAR Government, Research and laboratory work on the Chinese medicinal herb, namely Radix Paeoniae alba, $381,350
  • 2004-2005, Dept of Health, HKSAR Government, Research and laboratory work on the Chinese medicinal herb, namely Radix Aucklandiae, $381,350
  • 2004-2005, Dept of Health, HKSAR Government, Research and laboratory work on the Chinese medicinal herb, namely Radix Paeoniae rubra, $381,350
  • 2003-2007, Research Grants Council (RGC) Earmarked Grants,Gene expression during ovarian maturation in penaeid shrimp, $1,697,600
  • 2003-2005, CUHK Research Committee Funding (Direct Grants), Identification of genes related to selected agronomic traits of Shiitake mushroom, $22,157
  • 2002-2004, CUHK Research Committee Funding (Direct Grants), Molecular authentication of traditional Chinese medicinal plants, $29,612
  • 2002-2003, Dept of Health, HKSAR Government, Research and laboratory work on the Chinese medicinal herb, namely Cortex Phellodentri, $388,700
  • 2002-2003, Dept of Health, HKSAR Government, Research and laboratory, work on the Chinese medicinal herb, namely Rhizoma Alismatis (Alisma orientalis (Sam.) Juzep.), $388,700
  • 2002-2003, Dept of Health, HKSAR Government, Research and laboratory, work on the Chinese medicinal herb, namely Cortex Moutan (Paeonia suffruticosa Andr.), $388,700
  • 2002-2003, Dept of Health, HKSAR Government, Research and laboratory, work on the Chinese medicinal herb, namely Radix Astragali, $388,700
  • 2001-2004, Research Grants Council (Earmarked Grants), Identification and characterization of genes differentially expressed in dikaryotic mycelium of Shiitake mushroom Lentinula edodes by cDNA microarray hybridization, $771,100
  • 2001-2003, CUHK Research Committee Funding (Direct Grants), Isolation and differential expression analysis of hydrophobin genes of Shitake mushroom Lentinula edodes, $20,207
  • 2000-2006, University Grants Committee – Areas of Excellence (AoE), UGC-AoE plant and fungal biotechnology project, $38,823,000
  • 2000-2002, CUHK Research Committee Funding (Direct Grants), Analysis of mitogen activated protein kinase (MAPK) cascades isolated during fruit body development of Shiitake mushroom Lentinula edodes using yeast two-hybrid system, $100,000
  • 1999-2003, CUHK Strategic Research Program, Germplasm bank, chemical and molecular characterization of Chinese medicinal plants commonly used in Hong Kong, $500,000
  • 1998-2001, Research Grants Council (Earmarked Grants), Elucidation of gene expression profiles during fruit body development of Shiitake mushroom Lentinula edodes using expressed sequence tags and serial analysis of gene expression, $600,000
  • 1998-2001, Research Grants Council (Earmarked Grants), Development of molecular markers for genome napping in penaeid shrimp, $600,000
  • 1998-2000, Industrial Support Fund, Industry & Technology Development Council, Development of over-the-counter pharmaceutical products based on local medicinal plants, $8,128,000
  • 1997-2000, Research Grants Council (Earmarked Grants), Yeast complementation analyses of signal transduction genes isolated from early fruit body development of Shiitake mushroom Lentinula edodes, $780,840
  • 1997-2000, Research Grants Council (Earmarked Grants), Molecular studies on shellfish allergens: cloning, sequencing, expression and immunological responses, $686,880
  • 1996-1999, Research Grants Council (Earmarked Grants), Development of a species genetic linkage map of the Shiitake mushroom Lentinula edodes with sequence-tagged-site, expressed-sequence-tag, and gene markers, $692,000
  • 1996-1997, CUHK Research Committee Funding (Direct Grants), Molecular analysis of anaerobic ribonucleotide reductase, nrdD, in Salmonella typhimurium, $40,000
  • 1995-1997, Research Grants Council (Earmarked Grants), Molecular cloning and time course studies of genes specially expressed during fruit body development in Shiitake mushroom Lentinula edodes, $713,000
  • 1995-1996, CUHK Research Committee Funding (Direct Grants), Molecular cloning of genes specifically expressed in dikaryon of shiitake mushroom Lentinula edodes by RNA fingerprinting with arbitrarily primed polymerase reaction, $30,250
  • 1994-1997, Research Grants Council (Earmarked Grants), Identification and molecular cloning of differentially expressed genes involved in lignocellulose utilization in shiitake mushroom Lentinula edodes, $583,000
  • 1994-1996, The Croucher Foundation, Molecular cloning and expression of crustracean allergens, $600,000
  • 1994-1995, CUHK Research Committee Funding (Direct Grants), A study on the post-harvest storage of straw mushroom, Volvariella volvacea, $38,000
  • 1994-1995, UK/Hong Kong Joint Research Scheme, the British Council, Regulation of cellulase genes in the oyster mushroom Pleurotus, $80,352
  • 1993-1996, Research Grants Council (Earmarked Grants), Generation of molecular markers for construction of a genetic map, and characterization of genetic determinants of some phenotypic traits in the shiitake mushroom Lentinula edodes, $756,000
  • 1993-1994, The Croucher Foundation, Developing a gene transfer system for Pleurotus and its application by controlled expression of cellulase genes, for the improved utilization of cellulosic wastes for mushroom production, $35,000
  • 1993, FAO of United Nations, Draft proposal for support to inter-country ad hoc meeting on collaborative mushroom research and development, $78,000
  • 1992-1994, CUHK Research Committee Funding (Direct Grants), Cloning of DNA coding for shrimp allergens, $100,000
  • 1991-1992, CUHK Research Committee Funding (Direct Grants), Integrative biotechnological development of edible mushrooms as health food, $45,000
  • 1990-1993, The Croucher Foundation, Establishment of the genomic library, linkage map and germplasm bank for Lentinula edodes, $627,000
  • 1989-1993, United College Endowment Fund, CUHK, Electrophoretic karyotype analysis for mushroom species, Coprinus cinereus, Pleurotus ostreatus, Schizophyllum commune, and Lentinus edodes, $20,000
  • 1989-1992, Research Grants Council (Earmarked Grants), Ethanol production by anaerobic fermentation in genetically manipulated terrestrial and marine enteric bacteria, $250,000
  • 1989-1992, The Croucher Foundation, Sequencing of the adhR gene, $60,000
  • 1989-1990, CUHK Research Committee Funding (Direct Grants), Development of the molecular biological technique single primer polymerase chain reaction, $40,000
  • 1988-1991, Research Grants Council (Earmarked Grants), Removal of heavy metals from wastewater by immobilized microbial cells, $500,000

Teaching Development Grant (教學方面)

  • 2008-2011, Quality Education Fund, HKSAR Government, The development of student-oriented teaching and learning resources in science and a science enrichment programme for secondary 3-4 Students, $ 3,500,000
  • 2008, Education Bureau, Teacher Professional Development Programme- knowledge enriching series for new senior secondary technology and living: food science and technology strand, $1,280,000
  • 2007-2008, Education and Manpower Bureau, HKSAR Government, Development of learning and teaching resource packages for the new senior secondary “Technology and Living” curriculum, $600,000
  • 2006-2008, Quality Education Fund, HKSAR Government, A science enrichment programme for secondary 3-4 Students, $2,000,000
  • 2006-2008, UGC-funded Teaching Development Grant, Promoting the use of the outcome-based approach in science programmes: Development of learning outcome statements and measurement instruments, $800,000
  • 2005-2007,CUHK Teaching Development Grant, Engaging science students in the design and enactment of assessment, $160,000
  • 2004-2006, Quality Education Fund, HKSAR Government, Case-based learning of high school science subjects to support learning to learn, $2,820,000

Patents

Authenification of biologic materials using DNA-DNA hybridization on a solid support (principal investigator) (US patent #7297490, 2008)

Professional Activities

Membership in the following Professional Organizations:

Hong Kong Society of Microbiology (1984-1995)
American Society for Microbiology (1982-present)
American Association for Advancement of Science (1982-present)
World Society of Mushroom Biology and Mushroom Products (1993-1995)
Genetic Society of America (2007-present)
Coprinopsis cinerea Genome Consortium (2006-present)
Others

I have been very active in research. My research concentrates on the application of molecular biological technology in the studies of basic and applied biological problems.  For all my years in CUHK, I have been a major source of molecular technology in the Biology Department, the Science Faculty and CUHK as a whole.  Since 1993, the Biology Department has placed great emphasis in molecular biology and biotechnology leading to our success in the granting of an area of excellence in “Plant and Fungal Biotechnology”.  I have been contributing to the development of CUHK in this direction persistently and consistently for a long time.

I have been very successful in applying molecular technology in many fields to provide pioneering results in these fields.  Many of these research projects have been collaborations with other colleagues of the Biology Department or CUHK.  These collaborations often led to technology transfer from my laboratory to the collaborators’ laboratories.  My research interests are quite diverse but always incorporate the most recent and important research development:

  1. My group was the first group to perform polymerase chain reaction in Hong Kong, one of the first to sequence DNA, and the first to sequence genomes.
  2. I acquired the first genome sequencer and set up the genome sequencing service in Hong Kong.
  3. I have ample experience in collaborating with faculty members in many disciplines.  My collaborators include people in our university, from Biochemistry, Biology, Chemistry, Chinese Medicine, Mathematics, Statistics, and Medicine.
  4. My collaboration in mushroom biology research includes laboratories in USA and China.
  5. I am part of a global consortium working on genomics of the model mushroom Coprinopsis cinerea.
  6. My research in mushroom biology has international standing, as attested by many invited talks in international conferences.
  7. The community recognizes me as an expert in food safety and quality assurance, as I am now chairing both the Food Safety Expert Committee of Food and Environmental Hygiene Department and Accreditation Advisory Board of Hong Kong Accreditation Service.

I have obtained, as principle investigator or co-investigator, over HK$103 million of external competitive research funding, HK$44m as PI and HK$68 as co-I. I have been a team member in many important projects including the Area of Excellence “Plant and Fungal Biotechnology”. I am leading several substantial research projects including a substantial project “Construction of Food Composition Database for Nutritional Labelling”, which would benefit many small and medium enterprises in the Hong Kong food industry, and “First-Stage Development of a Comprehensive Genome Sequence Database for the Identification of Foodborne Pathogens in Hong Kong”, a project supported by the Research Fund for Control of Infectious Diseases, Food and Health Bureau. The spectrum of my research covers fundamental to applied research, from genomics to chemical analysis. My publications in mushroom genetics and genomics are considered to be pioneering works in mycology.